A US soldier, part of the Nato-led International Security Assistance Force (ISAF), stands outside a military base in Panjwai, Kandahar province south of Kabul. -AP Photo

BALANDI: An American soldier opened fire on villagers near his base in southern Afghanistan Sunday and killed 16 civilians, according to President Hamid Karzai who called it an ''assassination'' and furiously demanded an explanation from Washington. Nine children and three women were among the dead.

The killing spree deepened a crisis between US forces and their Afghan hosts over Americans burning Muslim holy books on a base in Afghanistan last month.

The burnings sparked weeks of violent protests and attacks that left some 30 dead. Six US service members have been killed by their Afghan colleagues since the Quran burnings came to light, but the violence had just started to calm down.

''This is an assassination, an intentional killing of innocent civilians and cannot be forgiven,'' Karzai said in a statement. He said he has repeatedly demanded the US stop killing Afghan civilians.

The violence over the Quran burnings has spurred calls in the US for a faster exit strategy from the 10-year-old Afghan war. President Barack Obama even said recently that ''now is the time for us to transition.'' But he also said he had no plan to change the current timetable that has Afghans taking control of security countrywide by the end of 2014.

The tensions between the two countries had appeared to be easing as recently as Friday, when the US and Afghan governments signed a memorandum of understanding about the transfer of Afghan detainees to Afghan control, a key step toward an eventual strategic partnership to govern US forces in the country. But Sunday's shooting could push that agreement further away.

''This is a fatal hammer blow on the US military mission in Afghanistan. Whatever sliver of trust and credibility we might have had following the burnings of the Quran is now gone,'' said David Cortright, the director of policy studies at Notre Dame's Kroc Institute for International Peace Studies and an advocate for a quick withdrawal from Afghanistan.

''This may have been the act of a lone, deranged soldier. But the people of Afghanistan will see it for what it was, a wanton massacre of innocent civilians,'' Cortright said.

One villager said 11 of those killed were members of his family, some of them women and children. An AP photographer saw 15 bodies between the two villages caught up in the shooting. Some of the bodies had been burned, while others were covered with blankets.

A young boy partially wrapped in a blanket was in the back of a minibus, dried blood crusted on his face and pooled in his ear. His loose-fitting brown pants were partly burned, revealing a leg charred by fire.

Villagers packed inside the minibus looked on with concern as a woman spoke to reporters. She pulled back a blanket to reveal the body of a smaller child wearing what appeared to be red pajamas. A third dead child lay amid a pile of green blankets in the bed of a truck.

Some villagers questioned whether a single soldier could have killed so many people. But a US official in Washington said the American, an Army staff sergeant, was believed to have acted alone and that initial reports indicated he returned to the base after the shooting and turned himself in.

Five people were wounded in the pre-dawn attack in Kandahar province, including a 15-year-old boy named Rafiullah who was shot in the leg and spoke to Karzai over the telephone. He described how the American soldier entered his house in the middle of the night, woke up his family and began shooting them, according to Karzai's statement.

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