Scores of people were injured on Saturday as Israeli forces targeted Palestinians outside the Old City of Jerusalem for the second night as tens of thousands of Muslim worshippers prayed at the nearby Al-Aqsa Mosque on Lailatul Qadr.

Palestinian youth threw stones, lit fires and tore down police barricades in the streets leading to the walled Old City gates as officers on horseback and in riot gear used stun grenades and water cannons to repel them.

At least 80 people were injured, including minors and a one-year-old, and 14 were taken to hospital, the Palestine Red Crescent said. Israeli police said at least one officer was hurt.

“They do not want us to pray. There is a fight every day, every day there are clashes. Every day there are troubles,” said Mahmoud al-Marbua, 27, speaking near the Old City's Damascus Gate.

Pointing to police chasing youths and firing thunder-flashes at them, he added: “Look how they are firing at us. How can we live?”

Tensions have mounted in the city throughout the Muslim holy month of Ramazan, amid growing anger over the potential eviction of Palestinians from Jerusalem homes on land claimed by Jewish settlers.

In the Palestinian Gaza Strip, hundreds of protesters gathered along the border with Israel. The Israeli military said the crowds threw burning tyres and firecrackers toward the troops.

Gaza militants fired at least one rocket into Israel which landed in an open area, the military said.

“We salute the ppl. of Al-Aqsa, who oppose the arrogance of the Zionists & we call on our ppl. in Palestine to support their brothers by all means,” Moussa Abu Marzouk, a leader of the armed Islamist group Hamas that rules Gaza, said on Twitter.

Israel said it was beefing up security forces on Saturday in anticipation of further confrontations in Jerusalem, the occupied West Bank and Gaza after fierce clashes erupted the previous night at Al-Aqsa Mosque.

A Palestinian official said Egypt was mediating between the sides to prevent further escalation and Saturday's violence appeared less pronounced than Friday's events.

On Friday, police fired rubber bullets and stun grenades towards rock-hurling Palestinian youths at the mosque on the Noble Sanctuary/Temple Mount plaza holy to both Muslims and Jews.

At least 205 Palestinians and 18 Israeli officers were injured in Friday's confrontations, which drew international condemnations and calls for calm.

Clashes have erupted nightly in East Jerusalem's Sheikh Jarrah — a neighbourhood where numerous Palestinian families face eviction in a long-running legal case. Police said dozens of protesters threw stones at officers there on Saturday.

Police Commissioner Yaakov Shabtai said extra officers were being deployed in Jerusalem on Saturday to “enable freedom of worship and maintain order and security”.

“At the same time, we will not allow violent riots, lawbreaking or the harming of police officers. We ask everyone to calm the spirits and violence, particularly on such an important day for the Muslim religion,” Shabtai said in a statement.

The Israeli military said it was boosting troops in the West Bank and near the Gaza Strip, where Palestinians have sent incendiary balloons over the border, igniting brushfires in Israeli territory. A military spokesman said extra forces there would largely be firefighting ones.

Quartet appeal

The Middle East quartet of mediators — the United States, Russia, the European Union and the United Nations — expressed concern over the violence and potential Jerusalem evictions.

“We call upon Israeli authorities to exercise restraint and to avoid measures that would further escalate the situation during this period of Muslim Holy Days,” the Quartet said in a statement.

Prime Minister Benjamin Netanyahu said in a statement that law and order would be maintained in Jerusalem as would the right to worship.

Television footage showed buses of Muslim worshippers heading from Israeli Arab cities to Al-Aqsa being stopped by police on the main highway to Jerusalem.

Word of the roadblock spread on social media, drawing hundreds of young men from nearby Arab villages and from Jerusalem.

Dozens drove their cars the wrong way down the now-empty Jerusalem-bound lanes, picking up fellow Muslims who had abandoned their own vehicles to start the uphill trek on foot. Some chanted in Arabic: “With our souls and our blood, we will redeem you, Al-Aqsa!”

Police said it was stopping only those planning to take part in riots before the buses were allowed to proceed. Scuffles broke out and footage showed officers firing stun grenades.

Tension was expected to remain high over the next few days. Israel's Supreme Court will hold a hearing on the Sheikh Jarrah evictions on Monday, the same day that Israel marks Jerusalem Day — its annual celebration of its capture of East Jerusalem during the 1967 Middle East war.

Palestinians want East Jerusalem as the capital of a future state. Israel claims the entire city as its eternal, indivisible capital. Its annexation of the eastern section was not recognised internationally.

PM Imran calls for immediate action

Prime Minister Imran Khan strongly condemned the attacks and said they violated "all norms of humanity and international law".

"We reiterate support for Palestinian people," he said and further called upon the international community to take immediate action for the protection of "Palestinians and their legitimate rights".

Meanwhile, President Dr Arif Alvi said it was a “shame that Israeli apartheid against Palestinians continues”.

Terming the attack on worshippers “atrocious”, Alvi asked the Palestinians to not lose hope. “Time is near when international politics will be based on morality [and] not on vested interests,” he tweeted.

"Pakistan has always strongly condemned Israeli attacks on Palestine," said Foreign Minister Shah Mehmood Qureshi in a statement, adding that there was no moral or political justification for the oppression and eviction of Palestinians.

He said that making worshippers involved in prayer on the night of 27th Ramazan the targets of violence was condemnable and that persecution of Palestinians was not acceptable in any situation.

Qureshi reiterated that Palestinians should get their rights and Pakistan stood with Palestine.

"Pakistan is a champion of the rights of Palestinians and has always taken a clear and firm stand on Palestine.

"I am glad that the Arab League has called an emergency meeting in this regard. I think the Arab League, the OIC (Organisation of Islamic Cooperation) and the Muslim world should work together to speak out against this barbarism and draw the attention of human rights activists [and] Western countries," said the foreign minister.

Additional reporting by Naveed Siddiqui.